| 273 | 0 | 70 |
| 下载次数 | 被引频次 | 阅读次数 |
为探究荒漠草原植物-真菌-土壤间的相互关系，了解内蒙古荒漠草原主要植物根际与非根际土壤真菌多样性以及形成差异的驱动因子，采用高通量测序技术，分析内蒙古荒漠草原群落中建群种短花针茅、优势种冷蒿和无芒隐子草根际土壤及非根际土壤真菌群落组成与多样性。结果表明，与非根际土壤相比，短花针茅根际土壤真菌多样性显著降低（P<0.05），冷蒿和无芒隐子草根际真菌多样性无显著变化。4组样品根际土壤共检测到5门26纲104目268科661属。在门水平，4组样品均包括Basidiomycota（担子菌门，73.39%～94.43%）、Ascomycota（子囊菌门，3.33%～12.41%）、Zygomycota（接合菌门，0.36%～1.45%）和Chytridiomycota（壶菌门，0.08%～1.59%）4门，短花针茅根际还有Glomeromycota（球囊菌门，0.6%）。属水平聚类分析表明，冷蒿和无芒隐子草根际真菌群落组成最相似。短花针茅根际的Inonotus、Coprinopsis、Bovista、Simocybe和Marasmius等真菌属的丰度显著高于其他3组，且LEfSe分析显示，短花针茅根际与其他3组的标志性差异真菌为Inonotus属。CCA分析结果表明，土壤pH和有机碳是影响真菌群落的主要环境因子。
Abstract：This study aims to explore the interrelationships among desert steppe plants, fungi, and soil and to understand the diversity of rhizosphere and non-rhizosphere soil fungi of dominant plants in the Inner Mongolia desert steppe and the driving factors behind the differences. High-throughput sequencing technology was employed to analyze the composition and diversity of fungal communities in rhizosphere and non-rhizosphere soils of constructive species Stipa breviflora and dominant species Artemisia frigida and Cleistogenes songorica in the Inner Mongolia desert steppe. The results showed that compared to non-rhizosphere soil, the S. breviflora rhizosphere soil had significantly decreased fungal diversity(P<0. 05), while rhizosphere soils of A. frigida and C. songorica did not show significant changes in fungal diversity. A total of 5 phyla, 26 classes, 104 orders, 268 families, and 661 genera were detected in the four groups of rhizosphere soil samples. At the phylum level, all the four groups included Basidiomycota(73. 39%-94. 43%), Ascomycota(3. 33%-12. 41%), Zygomycota(0. 36%-1. 45%), and Chytridiomycota(0. 08%-1. 59%). Additionally, Glomeromycota(0. 6%) was detected in rhizosphere soil of S. breviflora. Cluster analysis at the genus level revealed that the fungal community composition of A. frigida and C. songorica rhizosphere was most similar. The abundance of fungal genera such as Inonotus, Coprinopsis, Bovista, Simocybe and Marasmius in S. breviflora rhizosphere soil was significantly higher than that of the other three groups. LEfSe analysis indicated that Inonotus was the key differential fungi of S.breviflora rhizosphere from the other groups. CCA demonstrated that soil pH and organic carbon were the primary environmental factors influencing fungal communities.
[1] DOWLING J E, WALD G. Proceedings of the national academy of sciences of the United States of America[J]. Nutrition Reviews, 1981, 39(3):135-138.
[2]陈晓，刘勇，李国雷，等.土壤真菌研究方法及人为干扰对森林土壤真菌群落影响研究进展[J].世界林业研究，2011,24(5):7-12.
[3]戴雅婷，闫志坚，解继红，等.基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究[J].土壤学报，2017, 54(3):735-748.
[4] STEINAUER K, TILMAN D, WRAGG P D, et al. Plant diversity effects on soil microbial functions and enzymes are stronger than warming in a grassland experiment[J]. Ecology, 2015, 96(1):99-112.
[5] ZHALNINA K, LOUIE K B, HAO Z, et al. Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly[J]. Nature Microbiology, 2018, 3(4):470-480.
[6] LI J Y, CHEN Q F, LI Q, et al. Influence of plants and environmental variables on the diversity of soil microbial communities in the Yellow River Delta Wetland, China[J]. Chemosphere, 2021, 274:129967.
[7]高雪峰，韩国栋，张国刚.短花针茅荒漠草原土壤微生物群落组成及结构[J].生态学报，2017, 37(15):5129-5136.
[8]赵新风，徐海量，张鹏，等.养分与水分添加对荒漠草地植物群落结构和物种多样性的影响[J].植物生态学报，2014,38(2):167-177.
[9] BEIMFORDE C, FELDBERG K, NYLINDER S, et al. Estimating the phanerozoic history of the Ascomycota lineages:Combining fossil and molecular data[J]. Molecular Phylogenetics and Evolution, 2014, 78:386-398.
[10] ZHOU J, FONG J J. Strong agricultural management effects on soil microbial community in a non-experimental agroecosystem[J]. Applied Soil Ecology, 2021, 165:103970.
[11]姜雪薇，马大龙，臧淑英，等.高通量测序分析大兴安岭典型森林土壤细菌和真菌群落特征[J].微生物学通报，2021, 48(4):1093-1105.
[12] GE Z, LI S Y, BOL R, et al. Differential long-term fertilization alters residue-derived labile organic carbon fractions and microbial community during straw residue decomposition[J]. Soil and Tillage Research, 2021, 213:105120.
[13]周玉杰，李建华，张广宇，等.基于高通量测序的橡胶林土壤真菌多样性及群落组成分析[J].南方农业学报，2018,49(9):1729-1735.
[14]刘欢，董凯，仁增旺堆，等.藏沙蒿与多年生禾草混播对西藏沙化草地植被及土壤真菌群落特征的影响[J].草业学报，2023, 32(6):45-57.
[15]伍文宪，张蕾，黄小琴，等.川西北高寒牧区不同人工草地对土壤微生物多样性影响[J].草业学报，2019, 28(3):29-41.
[16]王幼珊，刘润进.球囊菌门丛枝菌根真菌最新分类系统菌种名录[J].菌物学报，2017, 36(7):820-850.
[17] RUDOLPH S, MACIá-VICENTE J G, LOTZ-WINTER H, et al. Temporal variation of fungal diversity in a mosaic landscape in Germany[J]. Studies in Mycology, 2018, 89:95-104.
[18]方圆，王娓，姚晓东，等.我国北方温带草地土壤微生物群落组成及其环境影响因素[J].北京大学学报（自然科学版），2017, 53(1):142-150.
[19] MAESTRE F T, DELGADO-BAQUERIZO M, JEFFRIES T C, et al. Increasing aridity reduces soil microbial diversity and abundance in global drylands[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(51):15684-15689.
[20] YANG Y, LI T, WANG Y Q, et al. Negative effects of multiple global change factors on soil microbial diversity[J].Soil Biology and Biochemistry, 2021, 156:108229.
[21]马晨鑫，俞琦，刘永杰，等.内蒙古露天煤矿排土场植物恢复根际土壤真菌群落特征[J].生态学报，2025,45(4):1974-1986.
基本信息:
中图分类号:S812
引用信息:
[1]徐嘉燊,阿拉木斯其其格,李嘉欣,等.内蒙古荒漠草原主要植物根际与非根际真菌群落研究[J].内蒙古师范大学学报(自然科学版),2025,54(03):286-293.
基金信息:
国家自然科学基金资助项目“荒漠草原群落中不同生态位植物根系分泌物的根际效应及其有效组分的筛选”（31660157）; 内蒙古师范大学2024年大学生创新创业训练计划资助项目“内蒙古荒漠草原植物根际促生菌（PGPR）的筛选及复合微生物菌肥的研究”（S202410135024）
2024-12-26
2024
2025-03-31
2025
2
2025-03-12
2025-03-12
2025-03-12